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Engineering en Evolutie van synthetische adeno-geassocieerd virus (AAV) gentherapievectoren via DNA Family Shuffling
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Immunologie et infection
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Journal JoVE Immunologie et infection
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
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Engineering en Evolutie van synthetische adeno-geassocieerd virus (AAV) gentherapievectoren via DNA Family Shuffling

28,105 Views

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21:55 min

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April 02, 2012

DOI:

10.3791/3819-v

DOI:
10.3791/3819-v

•

21:55 min
April 02, 2012

•

24 Views
, , , , , ,
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology,Heidelberg University, 2Department of Infectious Diseases, Virology,Heidelberg University

Summary

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We tonen de basistechniek om moleculair ingenieur en synthetische adeno-geassocieerde virale (AAV) gentherapievectoren evolueren via DNA-familie shuffelen. Bovendien geven we algemene richtlijnen en representatieve voorbeelden voor de selectie en analyse van individuele chimere capsiden met verbeterde eigenschappen op doel cellen in cultuur of in muizen.

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Cite this Article

Kienle, E., Senís, E., Börner, K., Niopek, D., Wiedtke, E., Grosse, S., Grimm, D. Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling. J. Vis. Exp. (62), e3819, doi:10.3791/3819 (2012).

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