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Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
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Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
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March 28, 2012
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We describe procedures for preparation and electrophysiological recording from brain slices that maintain the dorsal-ventral axis of the medial entorhinal cortex (MEC). Because neural encoding of location follows a dorsal-ventral organization within the MEC, these procedures facilitate investigation of cellular mechanisms important for navigation and memory.
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Parasagittal SlicesInvestigationDorsal-ventral OrganizationRodent Medial Entorhinal CortexComputation In The BrainNeuronsSynaptic InputsMembrane Ion ChannelsNeuronal FunctionTopographically Organized Neural CircuitsPosition Of Individual NeuronsInformation EncodingSensory And Cognitive CircuitsSpatial RepresentationsMedial Entorhinal Cortex (MEC)Grid-like Firing FieldsDorsal-ventral Axis
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Pastoll, H., White, M., Nolan, M. Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex. J. Vis. Exp. (61), e3802, doi:10.3791/3802 (2012).
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