/
/
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
13,657 Views
•
07:30 min
•
November 21, 2012
DOI:
Summary
Automatically generated
The amperometric technique measures dopamine release from a single cell by detecting the oxidative current produced by spontaneous dopamine oxidization. Simultaneous voltage clamp and amperometry methodology reveal the mechanistic relationship between the overall "activity" of dopamine transporter and the regulatory role of this activity on the reverse transport of dopamine.
Explore More Videos
Single Cell MeasurementDopamine ReleaseVoltage-clampAmperometrySynaptic CleftPre-synaptic TargetsPost-synaptic TargetsDiffusionExtracellular EnzymesMembrane TransportersDopamine TransporterPeri-synaptic CleftInward Dopamine FluxUptakeOutward TransportEfflux Of DopamineSulzer Et Al.Forward ActivityReverse ActivityExtracellular Dopamine HomeostasisPatch Clamp RecordingAmperometric RecordingMillisecond Time ResolutionMembrane Potential Control
Read Article
Cite this Article
Saha, K., Swant, J., Khoshbouei, H. Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry. J. Vis. Exp. (69), e3798, doi:10.3791/3798 (2012).
Copy