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Genoverførsel til udviklingslandene Mouse indre øre af<em> In Vivo</em> Elektroporation
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Chapitres
- 00:05Titre
- 01:58Part 1: Ventral Laparotomy
- 05:23Ventral Midline Incision
- 07:46Part 2: Transuterine Microinjection
- 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
- 10:19Embryo Reorientation for Otocyst Injections
- 13:48Transuterine Microinjection
- 16:31Part 3: In vivo Electroporation
- 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
- 19:47Prophylactic Analgesia
- 20:18Surgical Subject the Morning after Surgery
- 20:55Part 4: Representative Results
Musen indre øre er et placode-afledt sanseorgan, hvis udviklings-program er udarbejdet under svangerskabet. Vi definerer en<em> In utero</em> Genoverførsel teknik bestående af tre trin: mus ventrale laparotomi, transuterine mikroinjektion, og<em> In vivo</em> Elektroporering. Vi bruger digital video mikroskopi at påvise de kritiske eksperimentelle embryologiske teknikker.
Tags
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
