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从查明的神经元和取食行为的钙信号的同时记录果蝇</em
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
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从查明的神经元和取食行为的钙信号的同时记录果蝇</em

DOI:
10.3791/3625-v

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06:55 min

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April 26, 2012

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1Department of Neurobiology,University of Massachusetts Medical School

Chapitres

  • 00:05Titre
  • 01:30Constructing the Fly Brain Live Imaging and Electrophysiology Stage or “FLIES”
  • 02:00Preparing the Fly for Observation
  • 04:41Stimulating the Proboscis
  • 05:28Simultaneous Recording of the Proboscis Extension Reflex and Calcium Imaging
  • 06:22Conclusion

Summary

Traduction automatique

Traduction automatique

果蝇，<em>果蝇</em>，延长喂养它的长鼻，从长鼻或睑板糖刺激。我有长鼻延伸（PER）的响应与钙成像技术相结合的意见，使我们能够监测活动在大脑中的神经元，同时与行为观察。

Tags

Simultaneous RecordingCalcium SignalsIdentified NeuronsFeeding BehaviorDrosophila MelanogasterNeuronal ActivityBehavior AnalysisGenetically Encoded Calcium IndicatorsGCaMP1Optogenetic TechniquesThermogenetic TechniquesActivating Identified NeuronsCritical Neurons For Feeding BehaviorProboscis Extension ResponsePERSweet StimulusSensory CellsSuboesophageal GanglionSOGBehavioral ObservationExperimental System

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