/
/
Dissektion og Kultur af Chick Statoacoustic Ganglion og rygmarven eksplanteret i Kollagen Gels for neurite udvækst Analyser
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 01:10Bead Preparation
- 01:52Statoacoustic Ganglion (SAG) Dissection
- 03:20Spinal Cord Dissection
- 05:28The Explant Culture with Either Purified Proteins or Protein-coated Beads
- 07:53Visualizing of Neurite Outgrowth by Immunohistochemistry
- 09:02Representative Results from Neurite Outgrowth Assays
- 10:42Conclusion
Vi viser, hvordan du dissekere og kultur chick E4 statoacoustic ganglion og E6 rygmarven eksplanteret. Eksplanteret dyrkes under serumfrit betingelser i 3D kollagen geler i 24 timer. Neurite lydhørhed er testet med vækstfaktor-suppleret medium og med protein-belagt perler.
Tags
DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
