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Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
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Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
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08:08 min
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December 08, 2011
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We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.
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Cytosolic Ca2+Isolated Contractile LymphaticsLymphatic VesselsFluid HomeostasisLipidsCentral CirculationSurveillance SystemAntigensMucosal ImmunityAdaptive Immune ResponsesInterstitial FluidInitial LymphaticsPressure GradientCollecting LymphaticsLymphangionsBicuspid ValvesBackflow PreventionContractile CyclePhasic Contractile PatternCardiac CycleSystolic PhaseDiastolic PhaseContraction FrequencyLymphatic Smooth MuscleTone GenerationMyogenic ConstrictionMyogenic DilationLuminal Pressure Increases And DecreasesMolecular MechanismsCytosolic Ca2+ Concentration
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Souza-Smith, F. M., Kurtz, K. M., Breslin, J. W. Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics. J. Vis. Exp. (58), e3438, doi:10.3791/3438 (2011).
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