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Organotypic Slice kulturer av embryonale Ventral midthjernen: Et system for å Study dopaminerge neuronal Development In vitro</em
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Journal JoVE Neurosciences
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Organotypic Slice kulturer av embryonale Ventral midthjernen: Et system for å Study dopaminerge neuronal Development In vitro</em

DOI:
10.3791/3350-v

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07:33 min

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January 31, 2012

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,
1Institute of Reconstructive Neurobiology,University of Bonn

Chapitres

  • 00:05Titre
  • 01:00Preparation for Dissection
  • 03:00Embryonic Brain Preparation and Vibratome Sectioning
  • 05:09Slice Culture and Time-lapse Imaging
  • 06:21Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
  • 07:04Conclusion

Summary

Traduction automatique

Traduction automatique

En metode for å generere organotypic skiver fra E12.5 murine embryonale midthjernen er beskrevet. Den organotypic slice kulturer kan brukes til å observere atferden til dopaminerge nevroner eller andre ventrale midthjernen nerveceller.

Tags

Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position

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