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Immunology and Infection
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JoVE Journal Immunology and Infection
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
 

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

Article DOI: 10.3791/3296-v • 15:01 min • September 24th, 2011
September 24th, 2011 •
1, 1, 1
1Department of Pharmacology, University of Saskatchewan

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résumé
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We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.

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Tracking Neutrophil Intraluminal Crawling Transendothelial Migration Chemotaxis Tissue Intravital Video Microscopy Leukocytes Inflammation Postcapillary Venules Luminal Surface Rolling Leukocytes Endothelium Firm Adhesion Chemokine Chemoattractants Venular Surface Intraluminal Crawling Transmigration Extravascular Tissue Chemoattractant Gradient Brightfield Intravital Microscopy Mouse Cremaster Muscle Neutrophil Recruitment Agarose Gel
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