Journal
/
Neurosciences
/
Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

DOI:
10.3791/3143-v

•

09:02 min

•

November 11, 2011

•

,
1Membrane Biology Group, Centre for integrative Physiology,University of Edinburgh

Chapitres

  • 00:05Titre
  • 02:02Experimental Setup
  • 02:36Sample Preparation
  • 04:32S1 Phase
  • 06:12Data Analysis

Summary

Traduction automatique

Traduction automatique

A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.

Tags

Quantitative AnalysisSynaptic Vesicle Pool ReplenishmentCultured Cerebellar Granule NeuronsFM DyesNeurotransmitter ReleaseEndocytosisRecycling PoolReadily Releasable PoolReserve PoolAssaySV TrafficClathrin-dependent EndocytosisActivity-dependent Bulk EndocytosisMobilisation MechanismsSV TurnoverCentral Nerve TerminalsHydrophobic Hydrocarbon TailHydrophilic Head GroupFluorescent Probes

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Read Article