This content is Free Access.
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
21,012 Views
•
10:24 min
•
October 05, 2011
DOI:
Tags
TransfectingNucleofectingInduced Pluripotent Stem CellsGenetic ModificationStem Cell BiologyClinical ApplicationsGene Delivery MethodsHESCsHuman Embryonic Stem CellsHuman Induced Pluripotent Stem CellsIPSCsPlasmidEnhanced Green Fluorescence ProteinEGFP ReporterFeeder-free CulturesFeeder Cell TransfectionStable Transgenic IPSC ClonesROCK InhibitorTrypsinized CellsFeedersFeeder Cell-conditioned MediumTransgene-expressing IPSCsAntibiotic SelectionStable Transgenic LinesPluripotency
Read Article
Cite this Article
Chatterjee, P., Cheung, Y., Liew, C. Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells. J. Vis. Exp. (56), e3110, doi:10.3791/3110 (2011).
Copy