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Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection
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Neurosciences
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Journal JoVE Neurosciences
Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection

DOI:
10.3791/3080-v

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11:12 min

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June 04, 2011

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1Department of Biological Sciences,Florida Atlantic University

Chapitres

  • 00:05Titre
  • 00:16Introduction
  • 00:47Adult Dissection
  • 03:04Dye Fill Procedure
  • 07:19Dye Fill Set-up
  • 10:06Possible Applications, Difficulties, and Modifications

Summary

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An in vivo dissection of the adult Drosophila ventral nerve cord (VNC) is demonstrated. This particular dissection method causes little damage to the VNC allowing the subsequent labeling of the giant fiber neurons with fluorescent dye for high resolution imaging.

Tags

Axonal MorphologyDendritic MorphologyNeuronal StructuresLabelingDissection MethodNervous SystemVentral Nerve CordCervical ConnectiveGiant FibersFluorescent DyeInjectionStainingImaging

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