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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
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Biologie
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Journal JoVE Biologie
Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

DOI:
10.3791/3030-v

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08:46 min

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September 29, 2011

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, ,
1Andrus Gerontology Center, Department of Biological Sciences, Department of Molecular and Computational Biology,University of Southern California, Los Angeles

Chapitres

  • 00:05Titre
  • 00:57Yeast Chronological Lifespan(CLS) in Liquid Culture
  • 02:16In situ Viability Assay
  • 03:32DNA Damage and Mutation Assays
  • 06:27Results of Life Span and DNA Mutation Assays
  • 08:26Conclusion

Summary

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Traduction automatique

Here we describe a set of DNA mutation assays that can be combined with the yeast chronological life span model to study the genes/pathways that regulate or contribute to genomic DNA instability during aging.

Tags

Age-dependent Genomic InstabilityS. CerevisiaeChronological Lifespan ModelSaccharomyces Cerevisiae Aging ModelLife Span Regulatory PathwaysDNA DamageGenome MaintenanceDiseases During AgingAge-dependent DNA MutationsBase SubstitutionsFrame-shift MutationsGross Chromosomal RearrangementsHomologous RecombinationHomeologous RecombinationNuclear DNA Repair ActivityYeast Chronological Life SpanDNA Damage AssaysMutation AssaysGenes/pathways That Regulate Genomic InstabilityAge-dependent DNA MutationsCancer In Mammals

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