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Immunologie et infection
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Desarrollo de programas específicos de células de tipo anti-gp120 del VIH aptámeros para la entrega de siRNA
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Immunologie et infection
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Journal JoVE Immunologie et infection
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Desarrollo de programas específicos de células de tipo anti-gp120 del VIH aptámeros para la entrega de siRNA

19,335 Views

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13:47 min

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June 23, 2011

DOI:

10.3791/2954-v

DOI:
10.3791/2954-v

•

13:47 min
June 23, 2011

•

5 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Summary

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Varios fluoro-2'-aptámeros de ARN del VIH-1BA-L con gp120 nanomol afinidad están aisladas de la biblioteca de ARN por<em> In vitro</em> Procedimiento SELEX. Un nuevo doble función inhibitoria anti-gp120 aptamer-siRNA quimera se crea y se muestra muy prometedora para sistémica anti-VIH.

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Zhou, J., Li, H., Zhang, J., Piotr, S., Rossi, J. Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. (52), e2954, doi:10.3791/2954 (2011).

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