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The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
Chapitres
- 00:05Titre
- 01:16Preparations: The Microscope Stage and PSS Solutions
- 02:53Preparation for Surgery
- 03:41Surgical Preparations of the Open Cremaster Muscle
- 06:16Intravital Imaging of the Cremaster Muscle Microcirculation
- 06:50Conclusion
A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.
