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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Journal JoVE Biologie
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

DOI:
10.3791/2813-v

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14:22 min

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July 16, 2011

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, ,
1The Sprott Center for Stem Cell Research, Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa

Chapitres

  • 00:05Titre
  • 02:17Ex vivo Erythropoiesis of Human Hematopoietic Stem/Progenitor Cells
  • 06:40Lentiviral Mediated Knockdown During ex vivo Erythroid Differentiation
  • 10:19Results of ex vivo Erythropoiesis of Human Hematopoietic Stem Cells
  • 13:37Conclusion

Summary

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An ex vivo protocol to generate mature human red blood cells from hematopoietic stem/progenitors is described. Additionally we describe an efficient lentiviral-delivery method to knockdown the transcription factor TAL1 in primary erythroid cells. The efficiency of lentivirus mediated gene delivery is demonstrated using GFP expressing viruses.

Tags

Lentiviral-mediated KnockdownEx Vivo ErythropoiesisHuman Hematopoietic Stem CellsCell DifferentiationGene ExpressionTranscription FactorsLineage-specific GenesCell LinesErythroid CellsStages Of DifferentiationPrimary HSCsErythroid DifferentiationCord BloodBone MarrowAdult Peripheral Blood

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