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קביעת מיטוכונדריאלי המינים ממברנה פוטנציאליים הריאקטיבי חמצן בנוירונים חולדה חיה קליפתיים
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Chapitres
- 00:05Titre
- 01:46Introduction
- 02:23Preparing the Stock Solutions for the Fluorescent Probes TMRM and H2DCF-DA
- 03:15Loading Rat Cortical Neurons with TMRM and H2DCF-DA
- 04:42Live Imaging of Neurons Incubated with TMRM to determine ΔΨm
- 06:17Live Imaging of Neurons Incubated with H2DCF-DA to Determine ROS
- 07:18Data Analysis
- 08:22Representative Results: Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
- 09:33Conclusion
Tags
Mitochondrial Membrane PotentialReactive Oxygen SpeciesLive Rat Cortical NeuronsRespiratory ChainATP GenerationOxidative StressEnvironmental ToxinsOxidative PhosphorylationMitochondrial DysfunctionFluorescent ProbesRhodamine 123TMRMTMREJC-1Fluorescence IndicatorsDihydroethidiumDihydrorhodamine 123H2DCF-DAFluorescence Intensity
