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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
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Chapitres
- 00:05Titre
- 02:13Preparing for Slicing and Culturing
- 03:17SCN Slicing Procedure
- 06:06Organotypic SCN Culture
- 08:09Results: Circadian Bioluminescence in SCN Slices
- 09:27Conclusion
The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.
Tags
Slice PreparationLuciferase RecordingClock Gene ActivitySuprachiasmatic NucleusCircadian ClockPhysiologySciences du comportementRetinaOptic NerveNegative Feedback LoopsClock GenesPeriod GenesCore ClockMembrane DepolarizationCalciumCAMPOscillationsGene ExpressionMetabolic ActivityElectrical ActivityIn Vitro StudyMolecular InvestigationsElectrophysiological InvestigationsMetabolic Investigations
