Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays
Transcription
After a collective 18 days of culture, wash the MEA culture with the extracellular solution prepared earlier at room temperature. Then, dry the contacts of the MEA chip with a piece of tissue, and mount the MEAs on the MEA holder. Finally, install the MEA on the recording setup.
Afterward, add 300 microliters of the extracellular solution, and wait for 10 minutes to allow the system to stabilize before recording. Now, record the spontaneous activity for two minutes from all electrodes, and identify the active electrodes. Then, identify the electrodes responding to stimulation.
To exclude the stimulation artifact, stimulate from the same electrode 10 times. If the culture responds at least eight out of 10 times, it can be assumed as a positive response upon electrode-induced stimulation. To identify background noise, apply TTX to the culture at a concentration of one micromolar to block the voltage-gated sodium channels, and then record for two minutes.
