Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices
Transcription
Secure a mouse hippocampal-entorhinal cortex slice in a submerged recording chamber.
Continuously flow oxygenated ACSF at a constant temperature to maintain tissue viability.
Position a stimulation pipette filled with an electrolyte solution within the stratum radiatum of the hippocampal CA3 region, containing neurons that receive information from the entorhinal cortex.
Insert an ACSF-filled recording pipette into the stratum pyramidale of the CA1 region, consisting of neurons that form synapses with axonal projections from the CA3.
Using the stimulation pipette, apply an electric pulse to trigger action potentials in the presynaptic CA3 neurons.
The action potential induces the release of neurotransmitters, which bind to receptors on the postsynaptic CA1 neurons. The binding induces ion influx, leading to a change in the membrane potential.
The combined changes in the membrane potential of multiple postsynaptic CA1 neurons, termed the local field potential or LFP, are recorded extracellularly by the recording pipette.
