Microelectrode Array-Based Assessment of Neuronal Networks in Mouse Spinal Cord Slices
Transcription
Take a microelectrode array, or MEA, well, containing aCSF. Position a mouse spinal cord slice in the well and secure it with a weighted net.
Place the MEA well in a recording system. Under a microscope, ensure maximal contact between the MEA electrodes and the superficial dorsal horn or SDH, a region rich in interneurons.
Maintain a continuous aCSF flow to equilibrate the tissue.
Neurons communicate through action potentials. Sodium ion influx depolarizes the membrane, followed by potassium ion outflow that causes repolarization. The membrane briefly hyperpolarizes, preventing a new action potential until the resting potential is restored.
Record spontaneous neuronal activity from the MEA electrodes. Signals co-occurring across multiple electrodes indicate a network of synaptically linked neurons.
Introduce a potassium channel inhibitor to prolong depolarization that leads to increased action potential frequency and synchronous rhythmic activity across the network.
More electrodes showing coincident signals indicate the inhibitor-mediated synchronous activity.
