A Modified Roller Tube Method for the Long-Term Culture of Rodent Brain Slices
Transcription
Take brain slices obtained from a rodent brain. The slices consist of the hippocampus, which contains hippocampal neurons.
Transfer the brain slices to coverslips containing chicken plasma.
Apply plasma mixed with thrombin to the slices. Thrombin induces plasma coagulation, creating a biological scaffold that promotes adhesion of the slices to the coverslips.
Take flat-sided roller tubes with a hole on the flat side and an adhesive disc attached around the hole on the exterior of the tubes.
Position the coverslips onto the adhesive discs, ensuring that the brain slices face the interior of the tubes.
Add a neuronal culture medium to the tubes and flow in a carbon dioxide and air mixture.
Cap the tubes, insert them into a roller rack, and incubate them with a rolling motion.
Aeration provided by the rolling incubation and the medium's nutrients support the long-term culturing of the brain slices.
