Generation of Neuronal Cells from Blood-Derived Pluripotent Stem Cells
Transcription
Take a microplate with glass coverslips placed at the bottom of the wells.
Introduce a positively charged polymer to coat the coverslips and wash to remove any unbound polymer. Treat with an extracellular matrix protein to coat the coverslips and aspirate the unbound proteins.
The coating acts as a culture substrate that facilitates cell adhesion.
Add an induction medium to the wells and introduce a suspension of blood-derived pluripotent stem cells, or BD-PSCs. These cells, capable of differentiating into various cell types, are generated via reprogramming blood progenitor cells.
Incubate for the cells to adhere to the substrate. Growth factors and nutrients in the induction medium transform BD-PSCs into neural progenitor cells.
Introduce a differentiation medium containing neuronal growth factors and incubate, triggering differentiation of the neural progenitors into a neuronal phenotype, exhibiting extended cellular processes and cell-cell contacts.
