Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
Transcription
Take a pre-sterilized skin biopsy section obtained from a lamb's forehead in an antibiotic-free medium.
The section comprises an outer epidermis and an underlying dermis layer.
Remove the medium and use a buffer to wash the section.
Make a circular cut in the section and remove the top layer. This breaks the barrier, generating a wound bed in the skin.
Transfer the wounded sections onto a permeable membrane insert in a multiwell plate containing an antibiotic-free medium.
Introduce a suspension of pathogenic bacteria onto the wound bed and incubate.
During incubation, the wound bed provides a tissue surface for bacterial attachment and the nutrients to support bacterial growth.
This allows the multiplication and spreading of the bacteria across the wound bed, establishing an infection.
