This content is Free Access.
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Chapitres
- 00:02Introduction
- 00:36Critical Reagents
- 01:10Preparation of Gametocyte Culture
- 05:10Feeding Mosquitoes a Parasite-Containing Bloodmeal
- 10:14Dissecting Mosquito Midguts
- 12:41Estimating Oocyst Loads
- 13:28Conclusion
- 13:59End Credits
Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
