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Induction of Experimental Autoimmune Encephalomyelitis in a Mouse Model
Transcription
Begin by subcutaneously injecting 10- to 13-week-old female mice with 200 micrograms of myelin oligodendrocyte glycoprotein, emulsified in 200 microliters of complete Freund's adjuvant containing 400 micrograms of Mycobacterium tuberculosis. Immediately, and 24 hours later, intraperitoneally inject the mice with 0.2 micrograms of pertussis toxin in 200 microliters of PBS.
It is essential that mice have been adapted both to handling and the experimental environment before induction to avoid inducing stress, which can prevent the development of clinical symptoms.
One week after the injection, examine the mice daily for clinical symptoms as described in the table.
