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RNAi筛选参与宿主因素牛痘病毒感染使用果蝇细胞
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August 25, 2010
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在病毒感染的新型主机所涉及的因素可以识别通过细胞为基础的的全基因组功能RNAi筛选的损失。一<em>果蝇</em>细胞培养模型的易用性和效率的RNAi由于这种方法特别适合。在这里，我们展示了这种技术使用<em>牛痘</em>病毒为例。
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RNAi ScreeningHost FactorsVaccinia Virus InfectionDrosophila CellsViral PathogensPublic Health ThreatBioterrorismTherapeutics DevelopmentRNA InterferenceGenome-wide ScreensLoss-of-function ScreensMammalian VirusesMedical ImportanceAgricultural ImportanceVirus Infection StepsEntryTranslationRNA ReplicationCellular FactorsViral ReplicationHuman Cells Infected With VirusesAntiviral Therapeutics Development
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Moser, T. S., Sabin, L. R., Cherry, S. RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells. J. Vis. Exp. (42), e2137, doi:10.3791/2137 (2010).
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