Periodic Acid-Schiff Staining of Cells: An In Vitro Technique to Detect Glycogen Levels in Peripheral Blood Mononuclear Cells

In this step, place the slide on a flat surface. Apply 2 milliliters of Periodic Acid solution on the sample, and incubate for 5 minutes at room temperature. Then, rinse the slide several times with distilled water. Apply 2 milliliters of Schiff's reagent on the slide, and incubate at room temperature for 15 minutes.

Subsequently, wash the slide with distilled water for 5 minutes, and then, leave it to air-dry. Next, apply 100 microliters mounting media on the slide, and cover it with one large coverslip, or apply 50 microliters and use two small coverslips. After that, apply clear nail polish on the edges of the coverslip. Let them dry overnight.

Then, obtain images with the Binocular Light microscope using the 100X objective.