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Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
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Neurosciences
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Journal JoVE Neurosciences
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

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06:32 min

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September 01, 2010

DOI:

10.3791/2086-v

DOI:
10.3791/2086-v

•

06:32 min
September 01, 2010

•

2 Views
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1Department of Cell and Developmental Biology,Vanderbilt University

Summary

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This protocol describes the isolation, enrichment, and maintenance of medulloblastoma tumor stem cells derived from mutant mice with ectopic Sonic hedgehog pathway activity.

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IsolationEnrichmentMaintenanceMedulloblastoma Stem CellsBrain TumorsNeurosphere AssaysNeural Stem CellsTumorigenesisTumor ProgressionShh SignalingCerebellar DevelopmentCancer Stem Cells

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Huang, X., Ketova, T., LItingtung, Y., Chiang, C. Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells. J. Vis. Exp. (43), e2086, doi:10.3791/2086 (2010).

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