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迁移人T淋巴细胞分离，培养和分析<em在体外</em

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Immunologie et infection

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Journal JoVE Immunologie et infection

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

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迁移人T淋巴细胞分离，培养和分析<em在体外</em

DOI:

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08:39 min

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June 01, 2010

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Craig T. Lefort, Minsoo Kim

¹Center for Vaccine Biology and Immunology,University of Rochester

Chapitres

00:00Titre
00:37Introduction
01:31Isolation of Human T Lymphocytes
02:57Culture of Human T Lymphocytes
05:04In Vitro Lymphocyte Migration Assay
06:15Capturing an Image Sequence Using NIS Elements Software
07:20Representative Results
08:12Conclusion

Summary

Traduction automatique

T淋巴细胞的迁移过程中出现归巢淋巴器官，从血管中退出，进入周围组织。在这里，我们描述了一个可以用来分析T淋巴细胞迁移的协议<em>在体外</em>。

Tags

T Lymphocyte Isolation Culture Migration Analysis Integrins Ligands Extracellular Matrix Proteins Cell Leading Edge Uropod Leukocyte Recruitment Autoimmune Diseases Inflammatory Diseases In Vitro System Two-dimensional Substrate Intercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1 Stromal Cell-derived Factor-1 (SDF-1)Migratory Velocity Integrin Blockade Actomyosin Machinery

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