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Biologie du développement

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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

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Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

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Biologie du développement

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Journal JoVE Biologie du développement

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/200859-v

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01:30 min

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November 10, 2023

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Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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Zebrafish Wildtype Transgenic Larvae Dissociation HEPES-buffered E3 Medium Trypsin-EDTA Anesthesia Tricaine

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