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复制叉停滞了迎头RNA聚合酶直接重新启动
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
DOI:

07:27 min

April 29, 2010

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Chapitres

  • 00:00Titre
  • 01:16Introduction
  • 01:39Preparation of the Halted RNAP Elongation Complex
  • 03:07Preparation of the Downstream Replicaton Fork
  • 04:06Assembly of the Replisome and Initiation of Leading Strand Synthesis at the Replication Fork
  • 05:49Representative Results for the Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
  • 06:49Conclusion

Summary

Traduction automatique

复制体的命运碰撞与头部的RNA聚合酶(RNAP)是未知的。我们发现,碰撞后的头对RNA聚合酶的复制体摊位,但恢复后,取代了从DNA的RNA聚合酶的伸长。 MFD促进便利碰撞后的RNA聚合酶的位移复制重新启动。

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