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双体录音从促性腺激素释放激素(GnRH)神经元下丘脑薄片鉴定绿色荧光蛋白(GFP)
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
DOI:

09:30 min

February 23, 2010

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Chapitres

  • 00:00Titre
  • 00:36Introduction
  • 01:09Preparation of Hypothalamic Brain Slices
  • 03:12Preparation of Glass Pipettes
  • 03:57Current Clamp Recording
  • 07:25Validation of Cell-Attached Recording Method
  • 09:09Conclusion

Summary

Traduction automatique

在神经系统的活动往往需要在特定人群中的神经元的同步行动,从潜在的排放。例如,脉冲促性腺激素释放激素(GnRH)可能需要GnRH神经元之间的协调活动。我们提出我们的方法途径,可靠取得的弥漫性分布的GnRH神经元的同步电生理记录。

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