This content is Free Access.
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
21,810 Views
•
09:30 min
•
February 23, 2010
DOI:
Summary
Automatically generated
Activity in neuronal systems often requires synchronous action potential discharges from neurons within a specific population. For example, pulses of gonadotropin-releasing hormone (GnRH) likely require coordinated activity between GnRH neurons. We present our methodological approach for reliably obtaining simultaneous electrophysiological recordings from the diffusely distributed GnRH neurons.
Explore More Videos
Gonadotropin-Releasing Hormone (GnRH)Green Fluorescent Protein (GFP)Hypothalamic SlicesPituitary ReleaseLuteinizing Hormone (LH)Follicle-stimulating Hormone (FSH)Reproductive FunctionHypothalamusMedian EminenceHypophysiotropic Portal SystemAnterior Pituitary GlandGonadotropinsSystemic CirculationEpisodic PulsesPeptide ContentReleasable PoolNeurosecretionSingle Unit ActivityMulti-unit RecordingsLH ReleaseGnRH SomataFibers
Read Article
Cite this Article
Hemond, P. J., Suter, K. J. Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices. J. Vis. Exp. (36), e1678, doi:10.3791/1678 (2010).
Copy