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Rening av specifik cell Befolkningen efter Fluorescens Aktivt cellsortering (FACS)
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Rening av specifik cell Befolkningen efter Fluorescens Aktivt cellsortering (FACS)

71,283 Views

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15:29 min

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July 10, 2010

DOI:

10.3791/1546-v

DOI:
10.3791/1546-v

•

15:29 min
July 10, 2010

•

30 Views
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1Blood Research Institute,BloodCenter of Wisconsin

Tags

PurificationSpecific Cell PopulationFluorescence Activated Cell SortingFACSTechniquePhenotypeBulk MethodsPanningComplement DepletionMagnetic Bead SeparationAdvantagesHigh PurityIdentifying MarkerDifferential Marker DensityInternal StainingIntracellular Protein ExpressionGenetically Modified Fluorescent Protein MarkerSizeGranularityFluorescenceFluorescently-tagged Monoclonal AntibodiesNegative SelectionFlow CytometerSorting CapacitySoftware

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Basu, S., Campbell, H. M., Dittel, B. N., Ray, A. Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS). J. Vis. Exp. (41), e1546, doi:10.3791/1546 (2010).

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