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Immunologie et infection
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Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

DOI:
10.3791/1442-v

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08:45 min

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September 09, 2009

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, ,
1Boyce Thompson Institute for Plant Research, 2Plant Pathology and Plant-Microbe Biology,Cornell University

Chapitres

  • 00:05Titre
  • 00:08Introduction
  • 00:34Preparing for the PTI Assay
  • 03:26Induction of PTI
  • 05:20Challenge Inoculation
  • 06:32Scoring for the Breakdown of PTI
  • 07:33Representative Results
  • 08:12Conclusion

Summary

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A cell death-based assay for PTI in Nicotiana benthamiana plants is described.

Tags

AssayPathogen-Associated Molecular PatternPAMPTriggered ImmunityPTIPlantsPattern Recognition ReceptorsPRRsPlasma MembranesMicrobial FeaturesPathogen-associated Molecular PatternsFLS2-dependent PathwayFlg22Bacterial FlagellinVirulence FactorsEffectorsCell Death-based AssayN. BenthamianaModel SystemPlant-pathogen Interactions

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