Summary
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
Abstract
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
Protocol
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Divulgations
The authors have nothing to disclose.
Citer Cet Article
Centonze Frohlich, V. Proper Care and Cleaning of the Microscope. J. Vis. Exp. (18), e842, doi:10.3791/842 (2008).