A BW Reporter System for Studying Receptor-Ligand Interactions

Shlomo Elias; Shira Kahlon; Alexandra Duev-Cohen; Ofer Mandelboim

doi:10.3791/58685

JoVE Journal > Biology

Biologie

A BW Reporter System for Studying Receptor-Ligand Interactions

Published: January 07, 2019

doi:

10.3791/58685

Shlomo Elias^1,2, Shira Kahlon², Alexandra Duev-Cohen², Ofer Mandelboim²

¹Department of Hematology,Hadassah – Hebrew University Medical Center, ²The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah – Hebrew University Medical Center

Summary

This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions.

Abstract

Interactions between receptors and ligands constitute a fundamental biological process. However, direct experiments with cells that express the native receptor and the ligand are challenging since the ligand of a specific receptor may be unknown and experimental procedures with the native ligand can be technically complicated. To address these obstacles, we describe a reporter system to detect the binding and activation of a specific receptor by a ligand of interest. In this reporter system, the extracellular domain of a specific receptor is conjugated to mouse CD3ζ and this chimeric protein is then expressed in mouse BW cells. These transfected BW cells can then be incubated with different targets (e.g., cells or antibodies). Activation of a transfected receptor leads to the secretion of mouse interleukin-2 (mIL-2) which can be detected by enzyme-linked immunosorbent assay (ELISA). This reporter system has the advantages of being sensitive and specific to a single receptor. In addition, the activation level of a specific receptor can easily be quantified and can be used even in cases where the ligand of the receptor is unknown. This system has been implemented successfully in many of our studies to characterize receptor-ligand interactions. We have recently employed this system to study the activation of human Fcγ receptors (FcγRs) by different monoclonal anti-CD20 antibodies in clinical use.

Introduction

The BW reporter system is a technique for studying receptor-ligand interactions¹. This system is especially advantageous when the ligand of a specific receptor is unknown or experiments with the endogenous ligand are technically difficult. It can also be used for studying the binding of monoclonal antibodies to human FcγRs. The method is based on expressing a chimeric protein in mouse BW cells. This chimeric protein is composed of the extracellular domain of the receptor of interest fused to the transmembrane and intracellular domains of the mouse ζ chain. Binding of appropriate ligands of the receptor leads to secretion of mouse IL-2, which can be easily detected by ELISA, as illustrated in Figure 1. This method is sensitive and specific to an individual receptor, easy to operate, and highly reproducible. It can thus complement additional tools for studying receptor-ligand interactions. For example, it can be used to screen several cell lines for the presence of a ligand for a specific receptor (even if the ligand itself has not been identified) or to detect the activation of human FcγRs by monoclonal antibodies or by human serum containing anti-viral antibodies. Although primary immune cells express endogenous FcγRs, they are generally difficult to handle and usually express several Fc receptors.

We have used this system successfully in many of our studies to characterize receptor-ligand interactions. These include identifying haemagglutinin as the ligand of the NK cell receptor NKp46², identifying PVR and nectin-2 as ligands for human and mouse TIGIT³^,⁴, and showing that the fusobacterium nucleatum bacterium binds and activates human TIGIT⁵. In addition, BW cells that express Fc receptors have been used successfully to detect anti-viral antibodies in patients' sera⁶^,⁷^,⁸. Specifically, we recently established BW cells that express human FcγRs to detect differential FcR activation of anti-CD20 antibodies used for the treatment of chronic lymphocytic leukemia (CLL)⁹. Importantly, the results of the BW reporter system have been validated in complementary experiments.

Protocol

1. Generation of a Plasmid that Expresses the Chimeric Construct

NOTE: The aim is to generate a plasmid that expresses the extracellular domain of the receptor of interest fused to the transmembrane and intracellular domains of the mouse CD3ζ chain (Figure 2A).

Retrieve the sequence of the extracellular domain of the receptor of interest including the signal peptide. Obtain DNA material which is expected to express this receptor (e.g., cDNA or a plasmid).
Obtain DNA material that expresses the transmembrane and the intracellular domains of the mouse CD3ζ chain (e.g., cDNA or a plasmid).
Design the sequence of the fusion protein based on the general structure shown in Figure 2A. Make sure that the whole sequence is within the same codon reading frame. This sequence is used to design appropriate primers and verify the final product.
Design two PCR reactions to amplify each of the fragments of the fusion proteins separately (Figure 2B, 2C)¹⁰.
NOTE: The specific conditions for the PCR reactions (e.g., elongation time and annealing temperature) depend on the nature of the primers, which are designed for a specific receptor.
1. Amplify the extracellular segment of the receptor.
  1. Design a 5' primer that includes part of the signal peptide of the receptor, a Kozak consensus sequence, and an appropriate restriction site (Figure 2B).
  2. Design a 3' primer that flanks both parts of the fusion protein (Figure 2B). For example, for the initial design this 3' primer can include the last 20 base pairs of the extracellular segment of the receptor and the first 9 base pairs of CD3ζ segment.
    NOTE: There is no need to add a restriction site to the 3' primer since this primer will be used to generate the fused sequence, and is not used for ligation.
2. Amplify the CD3ζ segment.
  1. Design a 5' primer which flanks both parts of the fusion protein (Figure 2C). For example, for the initial design the 5' primer can include the last 9 base pairs of the extracellular segment of the receptor and the first 20 base pairs of the CD3ζ segment.
    NOTE: There is no need to add a restriction site to the 5' primer since this primer will be used to generate the fused sequence, and is not used for ligation.
  2. Design a 3' primer which includes the end of the CD3ζ sequence as well as an appropriate restriction site (Figure 2C).
3. Correct the primer sequences in each of these two reactions so that the annealing temperature is similar in each pair (note that the primer, which includes sequences of the two parts of the fusion protein, only anneals with part of the sequence in each reaction).
Perform an additional PCR in which a mixture of the PCR products of the first two reactions is used as the DNA template with the 5' primer of the extracellular receptor segment (Step 1.4.1.1) and the 3' of the CD3ζ segment (Step 1.4.2.2) (Figure 2D)¹⁰. This reaction should generate the final fused sequence.
Proceed as usual for cloning.
1. Ligate the final PCR product to the target cut vector (the target vector is usually pcDNA3 which expresses G418 and ampicillin resistance).
2. Transform the ligated vector into competent bacteria¹¹.
  1. Thaw 50 µL of the competent bacteria on ice.
  2. Add the ligated vector to the bacteria and incubate on ice for 20 min.
  3. Incubate at 42 °C for 45 s.
  4. Add 200 µL of LB without antibiotics.
  5. Incubate for 1 h at 37 °C with continuous shaking.
  6. Seed on an LB plate with the appropriate antibiotics (e.g., ampicillin solution with a final concentration of 0.1 mg/mL).
3. Collect and grow several bacterial colonies in 5 mL of LB (in 15 mL tubes).
4. The next day, extract plasmids with a mini prep kit.
5. Analyze the gene insert with restriction enzymes.
6. Sequence the relevant colonies and verify that the sequence and the reading frame are correct (as indicated in Step 1.3).
Transform the verified plasmid into competent bacteria¹¹.
1. Thaw 20 µL of the competent bacteria on ice.
2. Add 1 µL of the plasmid to the bacteria and incubate on ice for 20 min.
3. Incubate at 42 °C for 45 s.
4. Add 200 µL of LB without antibiotics.
5. Incubate for 1 h at 37 °C with continuous shaking.
6. Seed on a bacterial growth plate with the appropriate antibiotics (e.g., ampicillin solution with a final concentration of 0.1 mg/mL).
The next day, grow a bacterial colony in a large LB container with 0.1 mg/mL ampicillin (~250 mL).
The next day, perform maxi prep according to the specific instructions provided by the kit.
NOTE: For the electroporation procedure, a large quantity of plasmid is required, as detailed below.

2. Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells

NOTE: Different methods can also be employed for transfection (e.g., by lentiviral infection). Prior to the electroporation, perform ethanol precipitation of the DNA as described below. The next steps require sterile conditions.

The day before, prepare the BW5147 cells ("BW cells") for electroporation. Plate 10 plates (of 10 cm) with 10 mL of 100,000 BW5147 cells/mL (i.e., a total of 10 x 10⁶ cells) with RPMI supplemented with 10% fetal calf serum (FCS), 1% sodium pyruvate, 1% L-glutamine, 1% non-essential amino acids and 1% penicillin-streptomycin ("complete medium").
Place 100 µg of the pcDNA3 plasmid that expresses the fusion protein in a 1.5-2 mL tube and add 3 M sodium acetate at pH 5.3-5.5 to it. The volume of the sodium acetate should correspond to 0.1 (10%) of the volume of the 100 µg plasmid; titrate the pH of the sodium acetate with a pH meter by adding NaOH or HCl.
Add 2.5 volumes of 100% ethanol to the mixture of the plasmid and the sodium acetate as described in Step 2.2 (2.5x the total volume of plasmid and sodium acetate). Incubate overnight at -20 °C or for 2 h at 70 °C.
2.4. 24 h after the BW cells have been plated, collect all the BW cells (~100 mL) in 2 50 mL tubes. Centrifuge the cells for 5 min at 515 x g and discard the supernatant.
Re-suspend the pellet from both tubes in 25 mL of RPMI- in a single 50 mL tube. For example, re-suspend the pellet of one tube with 25 mL of RPMI- and then use this fluid to re-suspend the fluid in the other 50 mL tube so that the total pellet from the BW cells is re-suspended in 25 mL of medium in a single tube.
NOTE: The medium should be without additions, since the serum might interfere with electroporation.
Centrifuge the cells again for 5 min at 515 x g. Re-suspend the pellet in 1 mL of RPMI, and transfer the contents to a 0.4 cm cuvette on ice.
Centrifuge the plasmid that underwent ethanol precipitation (Step 2.3) for 30 min at 16,000 x g and 4 °C.
Wash once with 1 mL of 70% ethanol and centrifuge for another 20 min at 16,000 x g and 4 °C (to wash out the salt).
Remove all the ethanol and let the pellet dry slightly (i.e., in an open tube in the tissue culture hood). Re-suspend the pellet with 100 µL of RPMI- previously heated to 60 °C.
Add the re-suspended plasmid (Step 2.8) to the cells in the cuvette and incubate for 5 min on ice.
Electroporate the cells at 0.23 kV, 250 capacitation. This should take ~4 ms.
Transfer the electroporated cells to a 50 mL tube, add 50 mL of complete medium and centrifuge for 5 min at 515 x g.
Discard the supernatant and re-suspend the cells with 50 mL of complete medium.
Plate the electroporated cells in 24-well culture dishes (1 mL per well, for a total of ~50 wells) and incubate at 37 °C and 5% CO₂ for 48 h.
Select the transfected cells with antibiotics. After 48 h add 1 mL of complete medium supplemented with 10 mg/mL G418 to each well (at this stage the final volume in each well is 2 mL and the final concentration of G418 in each well is 5 mg/mL).
NOTE: If the plasmid contains a different antibiotic resistance, determine the antibiotic concentration required to kill the untransfected BW cells prior to this step.
Every 48 h, carefully discard 1 mL of the upper volume of each well without stirring the medium in the well (the BW cells tend to be at the bottom of the well). Add complete medium supplemented with 5 mg/mL G418 to each well, so that the final volume in each well is again 2 mL.
Examine the culture plates for cell growth in all wells regularly.
NOTE: A change in the color of the medium to yellow may help identify growing cells; however, at the beginning the medium will be yellow because of the G418 itself. Identify positive wells (wells with growing cells). These wells are G418 resistant and therefore are expected to express the fusion protein. This process usually takes ~3 weeks.

3. Verification of the Expression of the Transfected Receptor in Cells in the Positive Wells.

Stain the transfected BW cells with a specific antibody against the receptor which was transfected.
Compare the expression level of the receptor by flow cytometry to the expression of the control cells (untransfected BW cells).
After verification of one or more wells, use cells immediately for experimental purposes, grow in culture, or freeze for future applications.
Verify the expression of the transfected receptor before performing a new experiment. After a few weeks of growth, the cells with G418 with a stable receptor expression, G418 can be omitted from the culture medium.

4. Incubation of the Transfected BW Cells with Targets

NOTE: When using the transfected BW cells for the first time, it is preferable to test them on targets that express a known ligand of the receptor of interest or on plate-bound antibodies specifically directed against the receptor of interest (cross-linking experiments; see below, section 4.2.1.3).

Preferably, split the BW cells 24 h before the experiment (for example, by adding 10 mL of complete medium to 2 mL of cells in culture in a new 10 cm culture plate).
Incubate the transfected BW cells with their targets. Perform the experiment simultaneously on control BW cells that express an empty vector (or parental BW cells). 96F plates are preferable (but 96U plates can also be used). Perform the experiment in triplicate. Suspend all the cells in the complete medium.
1. Prepare the targets. The type of target varies as a function of the objective, and can be cells, cells that have been pre-incubated with antibodies, or antibodies alone. This system has also been used with bacteria as targets⁵.
  1. If the targets are dividing cells (i.e., cell lines), irradiate them at 6,000 rad prior to the assay. Place 50,000 of the target cells in a single well of a 96 plate (in a volume of 100 µL).
  2. For experiments with antibodies and BW cells that express human FcγRs, incubate the target cells with an antibody on ice in a 96 well plate (for example, 50 µL of the target cells and 50 µL of the antibody; different antibody doses can be tested).
  3. If using antibodies alone as targets (cross-linking experiments), they should be plate- bound. For this purpose, first incubate the antibodies in a complete medium in a 96F plate for 1-2 h at 37 °C and 5% CO₂ (a typical starting dose is 0.5 μg of a specific antibody in 50 μL). Wash the plate to remove unbound antibodies.
    NOTE: In this case, a 96F plate will enable binding of the antibody to the plate, which after addition of the transfected BW cells will lead to activation of the transfected receptor.
2. Add the BW cells (effector cells). Place 50,000 BW cells in a single well of a 96 plate (in a volume of 100 µL).
3. Complete the volume in each well to 200 µL if necessary.
4. Incubate the plates at 37 °C and 5% CO₂ for 48 h (this time period can be calibrated).
After 48 h, freeze the plates at -20°C and thaw prior to ELISA, or use immediately for ELISA (see next steps).

5. ELISA

Coat an ELISA plate with an anti-mouse IL-2 antibody (anti-mIL-2). Place 0.05 µg of anti-mIL-2 in a volume of 50 µL of 1x PBS per well. Incubate the coated ELISA plate with the anti-mIL-2 antibody at 4 °C overnight or at 37 °C for 2 h.
NOTE: To perform ELISA directly after the incubation step, the ELISA plate should be coated 24 h before the end of the incubation period of the BW cells.
Discard the fluid in the ELISA plate and add a blocking solution (200 µL per well) which is composed of 1x PBS and 1% bovine serum albumin (BSA). Incubate the ELISA plate for 2 h at room temperature.
Wash the ELISA plate three times with 0.05% PBS Tween solution (i.e., 0.5 mL of Tween-20 in 1 liter of 1x PBS).
Take the 96 plate which has the BW cells that were incubated with their targets (4.3). If the plate was frozen, completely thaw it (e.g., by short incubation at 37 °C). Centrifuge the 96 plate (5 min, 515 x g) and carefully transfer 100 µL of the supernatant in each well to the pre-coated and blocked ELISA plate.
NOTE: The supernatant should be collected from the sides of each well to avoid taking the cells.
If desired, add recombinant mIL-2 with defined concentrations to one of the empty rows of the coated ELISA plate to generate a standard curve of mIL-2. For example, start from a mIL-2 concentration of 2,500 pg/mL and then decrease by 50% in each subsequent well; do not add mIL-2 to the last well.
Incubate the ELISA plate at 4 °C overnight or at 37 °C for 2 h.
Wash the ELISA plate four times with PBS Tween.
Add biotin anti-mouse IL-2 to the ELISA plate. Use a concentration of 1 µg antibody in 1 mL of 1x PBS with 1% BSA and divide into 100 µL per well.
Incubate at room temperature for 1 h.
Wash the ELISA plate six times with PBS Tween.
Add HRP conjugated streptavidin. Use a concentration of 1 µL streptavidin in 1 mL of PBSX1 with 1% BSA and divide it into 100 µL per well.
Incubate at room temperature for 30 min.
Wash the ELISA plate six times with PBS Tween.
Add 100 µL per well of TMB substrate solution. Complete this stage quickly to minimize differences between the wells due to time lags when adding the TMB.
Read the ELISA plate with an ELISA plate reader at 650 nm (the reading can be repeated if the signal is weak).

Representative Results

Figure 3 illustrates the results of an experiment with a BW reporter system. In this experiment, CLL cells were pre-incubated with different anti-CD20 antibodies (rituximab and obinutuzumab) and then co-incubated with transfected BW cells which express CD16a-CD3ζ. Similar experiments were conducted in our study⁹. Figure 3A presents an image of a raw ELISA plate where the color intensity corresponds to a concentration of mIL-2. The experiment was performed in triplicate. This experiment included several controls: CLL cells incubated with parental untransfected BW cells (upper row) and CLL cells incubated with BW cells which express CD16a-CD3ζ but without an antibody or with a control antibody (six wells in the second row on the left). The incubation of BW cells that expressed CD16 with CLL cells that were pre-incubated with anti-CD20 antibodies induced a significant secretion of mIL-2 compared to the mIL-2 level of the control wells. Importantly, pre-incubation of the CLL cells with the anti-CD20 obinutuzumab activated CD16 more strongly than rituximab, a known finding which was recapitulated by our system. The last row shows descending pre-defined concentrations of recombinant mIL-2 (without the addition of cells). The right well in the last row does not have mIL-2 and represents the background reading, which appears similar to the control wells. Figure 3B presents the quantification of the results of the ELISA plate shown in Figure 3A. The optical density (OD) levels were converted into mIL-2 concentrations based on the standard curve with recombinant mIL-2. Figure 3C presents a dose response experiment where different doses of antibodies were pre-incubated with CLL cells and then incubated with BW cells expressing CD16.

Figure 1: Schematic representation of the BW reporter system. BW5147 cells are stably transfected with the extracellular portion of different receptors fused to the transmembrane and cytoplasmic domains of the mouse CD3ζ chain (chimeric recptor-CD3ζ). Activation of a specific receptor-CD3ζ by a ligand results in a secretion of mIL-2 which can be detected by ELISA. Please click here to view a larger version of this figure.

Figure 2: The structure of the chimeric receptor-CD3ζ and the design of PCR primers. (A) General structure of an extracellular receptor fused to the transmembrane and intracellular domains of mouse CD3ζ. (B) The extracellular domain of the receptor was amplified with a 3' primer which also included nucleotides of CD3ζ. (C) The intracellular and transmembrane domains of CD3ζ were amplified with a 5' primer that also included receptor nucleotides. (D) In the final PCR reaction, both segments, which have overlapping sequences, were used as the DNA template. In all figure panels, different protein domains are color-coded and boxed in blue rectangles (CD3ζ sequence) and red rectangles (the receptor sequence). Please click here to view a larger version of this figure.

Figure 3: Illustration of the final product of the BW reporter system. (A, B, C) CLL cells were pre-incubated with or without two anti-CD20 antibodies (rituximab and obinutuzumab) or a control antibody. Afterwards, the antibody-bound cells were incubated with parental BW cells or BW cells which express CD16a-CD3ζ. The level of mIL-2 in the supernatant was determined by ELISA. (A) An image of a raw ELISA plate. Color intensity indicates the concentration of mIL-2 in the supernatant. The experiment was performed in triplicate. The lower row shows the ELISA readout in decreasing concentrations of recombinant mIL-2 which were analyzed in the same plate. (B) Quantification of the ELISA reading shown in (A). The mIL-2 levels were calculated using a standard curve of mil-2. (C) A dose response experiment where different doses of antibodies were pre-incubated with CLL cells and then incubated with BW cells expressing CD16a-CD3ζ. ***, P < 0.001; Student's t test (B) or ANOVA with multiple comparisons (C). The only comparison in (C) which was not significantly different was for rituximab and the control Ab in the first concentration (0.01 µg/mL). Error bars represent the standard deviation of the triplicates. Similar experiments were conducted as part of one of our recent study⁹. Please click here to view a larger version of this figure.

Discussion

Here we present a protocol for generating a reporter system to investigate receptor-ligand interactions (see an abbreviated form of this protocol in¹). The protocol is composed of three main parts: cloning of a chimeric protein, which includes the extracellular domain of a specific receptor fused to the intracellular domain of CD3ζ, transfection of a plasmid that expresses the fusion protein to BW cells (e.g., by electroporation), and quantitative detection of mouse IL-2 by ELISA. The final product of each of these parts should be verified independently: the cloning process should be verified by complete sequencing of the fusion protein in the target plasmid, the transfection of the BW cells should be verified by examining the expression of the receptor of interest in the BW cells (e.g., by flow cytometry), and the ELISA process should be verified by general positive controls (e.g., recombinant mIL-2) as well as with specific controls for the transfected BW cells; i.e., targets that express a known ligand of the receptor of interest (e.g., cells that express CD48 can be used as a positive control for BW cells that express the NK cell receptor 2B4). For instance, the cloning process may be successful, but the fusion protein is not expressed by the BW cells. In this case, the electroporation should be repeated. Alternatively, if there is no signal above the background level of the ELISA plate (especially for the positive controls) the transfected BW cells may have failed to express the receptor (or the expression has been lost) or a technical problem may have occurred during the ELISA process.

Several modifications can be made to this protocol while preserving the general principles of the procedure. These modifications include the vector used to express the fusion protein, the method for transfecting the plasmid to BW cells and specific parameters related to the incubation and ELISA such as the type of target (e.g., cells, antibodies, etc.), number of target cells or the antibody concentration if relevant (we use 50,000 cells per well and an antibody starting dose of 0.5 µg/well), incubation time (we use an incubation time of 48 h) and the supernatant volume which is transferred to the ELISA plate.

This method is sensitive, specific and can be easily quantified. It is ideal for screening for the presence of ligands for specific receptors (especially if the ligand itself has not been recognized). After preparing transfected BW cells that express a specific receptor, these cells can be frozen and used when needed for additional experiments. We and others have used this system successfully in previous studies to explore receptor-ligand interactions (for example²^,³^,⁵^,¹²^,¹³^,¹⁴^,¹⁵^,¹⁶) and the results obtained by this system have been verified by other techniques. This method can also be used to study the activation of different human FcγR receptors by antibodies, as has been done for anti-viral antibodies in serum⁶^,⁷^,⁸ or with monoclonal antibodies against CD20⁹.

Since this is a reporter system in which only the extracellular segment of the receptor is expressed, the findings obtained by this system should be complemented by additional experiments with the endogenous receptor, such as cytotoxicity assays with natural killer (NK) cells to study interactions with NK cell receptors. Moreover, certain ligand/receptor interactions may not result in a mIL-2 secretion by the reporter system, and therefore might be overlooked. In addition, as in any experimental setup, the results should be verified for various parameters as described above. This is especially important in cases where a comparison of two conditions is required (e.g., activation of the same Fc receptor by different antibodies). It is also important to verify that the sensitivity of a specific ligand-receptor interaction is within the linear range of the system. Otherwise this could lead to saturation values which may preclude a valid comparison across conditions. This can be achieved by using a mIL-2 standard curve as well as by generating a dose-response curve with the tested ligand (in the case of saturating values, the experimental parameters should be modified; e.g., a smaller volume of supernatant should be analyzed by ELISA). Another possible limitation of this system is the use of target cells with endogenous secretions of mIL-2 (e.g., mouse T cells) which would mask the IL-2 secretion of BW cells. To overcome these highly unusual occurrences which are confined to mouse cells, this reporter system could be improved by using a different reporter (e.g., GFP) which is not expressed by the target cells.

Divulgations

The authors have nothing to disclose.

Acknowledgements

The authors thank Esther Singer for language editing. This study was supported by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement number 320473-BacNK. Further support was provided by the I-CORE Program of the Planning and Budgeting Committee and the Israel Science Foundation and by the I-Core on Chromatin and RNA in Gene Regulation, the GIF Foundation, the Lewis Family Foundation, the ICRF professorship grant, the Helmholtz Israel grant and the Rosetrees Trust (all to O.M.). This study was also supported by the Israel Science Foundation (grant 502/15), the Kass Medical Research Award and a research grant from the Israel Society of Hematology and Transfusion Medicine (to S.E). O.M is a Crown professor of Molecular Immunology.

Materials

AccuPrep, Plasmid Mini Extraction Kit	Bioneer	K-3030
Anti-mouse IL-2	BioLegend	503702
Biotin anti-mouse IL-2	BioLegend	503804
ELISA plates	De-groot	60-655061
Fetal Bovine Serum	Sigma	F7524-500ml
G418	Mercury	MBS3458105GM
Gene Pulser II	Bio-Rad	165-2105, 165-2106, 165-2107, 165-2108, 165-2109, 165-2110
L-Glutamine	Biological industry	03-020-1B
MEM Non-Essential Amino Acids Solution	Biological industry	01-340-1B
Penicillin-Streptomycin Solution	Biological industry	03-031-1B
peroxidase streptavidin	Jackson ImmunoResearch	016-030-084
PureLink HiPure Plasmid Filter Maxiprep Kit	ThermoFisher Scientific	K210016
RPMI-1640 Medium	Biological industry	30-2001
Sodium Pyruvate	Biological industry	03-042-1B
TMB	SouthernBiothech	0410-01

References

Mandelboim, O., Lankry, D., Gazit, R. . Natural Killer Cell Protocols. , 258-262 (2010).
Mandelboim, O., et al. Recognition of haemagglutinins on virus-infected cells by NKp46 activates lysis by human NK cells. Nature. 409 (6823), 1055-1060 (2001).
Stanietsky, N., et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proceedings of the National Academy of Sciences of the United States of America. 106 (42), 17858-17863 (2009).
Stanietsky, N., et al. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. European Journal of Immunology. , (2013).
Gur, C., et al. Binding of the Fap2 protein of Fusobacterium nucleatum to human inhibitory receptor TIGIT protects tumors from immune cell attack. Immunity. 42 (2), 344-355 (2015).
Corrales-Aguilar, E., et al. A novel assay for detecting virus-specific antibodies triggering activation of Fcgamma receptors. Journal of Immunoogical Methods. 387 (1-2), 21-35 (2013).
Corrales-Aguilar, E., et al. Highly individual patterns of virus-immune IgG effector responses in humans. Medical Microbiology and Immunology. 205 (5), 409-424 (2016).
Radinsky, O., et al. Sudan ebolavirus long recovered survivors produce GP-specific Abs that are of the IgG1 subclass and preferentially bind FcgammaRI. Scientific Reports. 7 (1), 6054 (2017).
Elias, S., Kahlon, S., Kotzur, R., Kaynan, N., Mandelboim, O. Obinutuzumab activates FcgammaRI more potently than other anti-CD20 antibodies in chronic lymphocytic leukemia (CLL). Oncoimmunology. 7 (6), e1428158 (2018).
Lorenz, T. C. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of Visualized Experiments. (63), e3998 (2012).
Froger, A., Hall, J. E. Transformation of plasmid DNA into E. coli using the heat shock method. Journal of Visualized Experiments. (6), 253 (2007).
Bar-On, Y., et al. NKp46 Recognizes the Sigma1 Protein of Reovirus: Implications for Reovirus-Based Cancer Therapy. Journal of Virology. 91 (19), (2017).
Glasner, A., et al. Expression, Function, and Molecular Properties of the Killer Receptor Ncr1-Noe. Journal of Immunology. 195 (8), 3959-3969 (2015).
Glatzer, T., et al. RORgammat(+) innate lymphoid cells acquire a proinflammatory program upon engagement of the activating receptor NKp44. Immunity. 38 (6), 1223-1235 (2013).
Gur, C., et al. The activating receptor NKp46 is essential for the development of type 1 diabetes. Nature Immunology. 11 (2), 121-128 (2010).
Markel, G., et al. Pivotal role of CEACAM1 protein in the inhibition of activated decidual lymphocyte functions. Journal of Clinical Investigation. 110 (7), 943-953 (2002).

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Elias, S., Kahlon, S., Duev-Cohen, A., Mandelboim, O. A BW Reporter System for Studying Receptor-Ligand Interactions. J. Vis. Exp. (143), e58685, doi:10.3791/58685 (2019).