A Mouse Aortic Interposition Model to Study Alloimmune-Induced Vascular Rejection

Published: May 31, 2024

Abstract

Source: Enns, W., et al. Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis. J. Vis. Exp. (2015).

This video demonstrates a method for generating a mouse aortic interposition model to study alloimmune-induced vascular rejection. After placing an anesthetized donor mouse in the supine position, the infrarenal abdominal aorta (IAA) is carefully isolated from surrounding structures. Branching vessels are ligated, and both ends of the IAA are clamped to prevent blood flow before resection. Subsequently, the donor IAA is implanted into an allogeneic recipient mouse, and blood flow is restored through anastomosis to investigate alloimmune-induced vascular rejection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. Use Balb/cYJ (H2d) donor mice and C57Bl/6 (H2b) recipient mice to examine allogeneic reactions. Mice are used for experiments between the ages of 8 to 12 weeks. Use either female or male mice. Syngeneic controls consist of aortic segments from C57Bl/6 donors into C57Bl/6 recipients. 1. Donor and …

Divulgations

The authors have nothing to disclose.

Materials

C57BL/6J (H-2b) Jackson Laboratories, Bar Harbour ME Strain# 000664
Balb/cBYJ Jackson Laboratories, Bar Harbour ME Strain# 001026
Ketamine Hydrochloride Injection USP 100 mg/ mL Ketalean DIN 00612316
Xylazine Injection 20 mg/mL Rompum DIN 02169592
Ketoprofen Injection 100 mg/mL Anafen DIN 01938126
Butorphanol Tartrate injection 10 mg/mL Torbugesic DIN 008450000
Buprenorphine Injection 0.3 mg/mL Reckitt Benckiser B.N. 5241
Atipamezole hydrochloride sterile injectable solution Antisedan DIN 02237744
Heparin Sodium Injection, USP, 1000 units/mL McKesson Distribution DIN 02264315
Tears naturale ophthalmic ointment Alcon DIN 02082519
Stereomicroscope Leica M80
0.9% Sodium Chloride, sterile Baxter Corporation
Lactated Ringer's solution, sterile Baxter Corporation
0.9% Sodium Chloride Injection, sterile, 10 mL Baxter Corporation
Alcohol Prep Pads Loris
Povidone Iodine Betadine
Chlorohexidine Gluconate 4% w/v Germi-Stat
Black Polyamide Monofilament AROSurgical Instruments T4A10Q07
Suture, 10-0 suture, 70 microns Corporation
Blue monofilament suture 5-0, P3 needle Ethicon 8698G
1 ml Syringe BD REF 309659
10 ml Syringe BD REF 309604
1cc TB insulin syringe with 28G 1/2 BD REF 309309
25G 7/8, hypodermic needle BD REF 305124
27G 1/2, hypodermic needle BD REF 305109
Colibri Retractor- 1.5cm spread 4cm Fine Science Tools 17000-04
S&T CAF-4 Clip applying forceps, without lock Fine Science Tools 00072-14
Supergrip forceps, S&T Fine Science Tools 00632-11
Medical No.5 forceps Fine Science Tools 11253-20
Lexer Baby Scissors Fine Science Tools 14078-10
Micro Adson forceps serrated Fine Science Tools 11018-12
Vannas-Tubingen microscissors Fine Science Tools 15003-08
Micro clamps, b-1; 3.5mm x 1mm; 7mm length Fine Science Tools 00396-01
Graefe-forceps, 10cm 1×2 teeth Fine Science Tools 11054-10
Castroviejo with lock and tungsten jaws Fine Science Tools 12565-14
Hot glass bead sterilizer Inotech 250 IS-250 – Steri-250
Non-woven gauzes Progene
Cotton Tipped Applicators Puritan
Beard Trimmer Wahl
Heating pad Sunbeam

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Citer Cet Article
A Mouse Aortic Interposition Model to Study Alloimmune-Induced Vascular Rejection. J. Vis. Exp. (Pending Publication), e22247, doi: (2024).

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