Targeting Liver Tumors with Oncolytic Viruses via the Hepatic Artery

Published: May 31, 2024

Abstract

Source: Altomonte, J. et al., Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats. J. Vis. Exp. (2016)

The video demonstrates locoregional delivery of oncolytic viruses through hepatic arterial injection as a therapeutic approach for hepatocellular carcinoma. These viruses selectively replicate in tumor cells, inducing cell lysis and releasing tumor cell-associated antigens. This process triggers immunogenic cell death, inducing an anti-tumor response.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparations before beginning surgery Sterilize surgical instruments (i.e., by autoclaving). Dilute the virus to the appropriate concentration, which will vary depending on the toxicity of the specific virus being used and the susceptibility of the rat strain. NOTE: For example…

Representative Results

Figure 1. Photographs of the hepatic arterial infusion procedure. A laparotomized rat demonstrates the placement of the abdominal wall retractor and gauze swabs (A). After lifting out the hepatic lobes, the common hepatic artery (designated CHA) and gastroduodenal artery (designated GA) are identified and dissected (B). After ligating the distal end of the gastro…

Divulgations

The authors have nothing to disclose.

Materials

Veterinary clippers Aesculap GT415 Small, cordless trimmer ideal for removing fur from surgical area
Stereomicroscope Zeiss Stemi SV6
30G Needles Braun 4656300 30G x ½"
1ml syringes Braun 9161406V Tuberculin syringe
Disposable scalpel Feather 2975#15 #15 blade
Standard surgical scissors Fine Science Tools 14001-13 Sharp/blunt, for opening skin and muscle
Adson forcep Fine Science Tools 1101-12 With teeth, for grasping skin and muscle
Alm retractor Fine Science Tools 17008-07 With blunt teeth, for spreading abdominal cavity open during surgery
Gauze swabs Lohmann & Rauscher 18504 7.5 x 7.5 cm, should be autoclaved prior to use
Cotton-tipped applicator swabs Lohmann & Rauscher 11970 Sterile
Fine-tipped foreceps Fine Science Tools 11063-07 0.4mm, angled tip, for dissecting hepatic artery
Vannas spring scissors Fine Science Tools 91500-09 For delicate cutting
Micro-needle holder Fine Science Tools 12076-12 For ligating gastroduodenal artery
Needle holder Fine Science Tools 12005-15 Tungsten carbide jaws
7-0 Prolene sutures Ethicon 8648H Polypropylene suture with curved needle, for ligating gastroduodenal artery
4-0 Vicryl sutures Ethicon V3040H With curved needle attached
Infrared warming lamp Beurer IL11 For maintaining body temperature post-operatively

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Citer Cet Article
Targeting Liver Tumors with Oncolytic Viruses via the Hepatic Artery. J. Vis. Exp. (Pending Publication), e22234, doi: (2024).

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