Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus

Published: April 30, 2024

Abstract

Source: Sajadi, A., et al. Neurodegeneration in an Animal Model of Chronic Amyloid-beta Oligomer Infusion Is Counteracted by Antibody Treatment Infused with Osmotic Pumps. J. Vis. Exp. (2016).

This video demonstrates a technique for the continuous infusion of amyloid-beta (Aβ) peptide-specific antibodies into the hippocampus of a rat. The study employs implanting a bilateral cannula system for a controlled infusion of Aβ peptide-specific antibodies coupled with the infusion of soluble Aβ oligomers (Aβo) into the rat hippocampus to study the interactions between the oligomers and the antibodies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Catheter Preparation before Stereotaxic Surgery Cut PE50 catheters (6 cm in length) that will be used to connect the cannula with osmotic pumps (see Figure 1). Fill PE50 catheters with artificial cerebrospinal fluid (aCSF) and seal both ends of the catheters. Keep the catheter at 4 °C until used.</…

Representative Results

Figure 1. Photo of the Animal with Bilateral Cannula During Aβo Infusion. A solution of Aβo (0.2 µg/µl; 1 µl) was injected in awake and freely moving rats (once a day for 6 days) using PE50 catheters connected to an internal cannula inserted into a guide cannula. Treatment with control (Ctl) IgG1 or 6E10 antibody was infused directly at the site of A&#946…

Divulgations

The authors have nothing to disclose.

Materials

Artificial Cerebrospinal Fluid (aCSF) Harvard Apparatus 59-7316
PE50 Catheter thin wall Plastics one C232CT
Ketamine Hydrochloride (100 mg/mL) Bioniche 1989529
Xylaxine Hydrochloride (100 mg/mL) Bimeda 8XYL004C
Meloxicam (5 mg/mL) Norbrook 215670I01
Solution of chlorhexidine gluconate 2% and isopropyl alcohol 2% Carefusion 260100C
Lidocaine Hydrochloride Alveda Pharma 0122AG01
Bupivacaine Hydrochloride Hospira 1559
Ophthalmic ointment Baussh and Lomb inc. 2125706
Stereotaxic frame Stoelting 51600
Stereotaxic cannula holder arm Harvard Apparatus 72-4837
Drill Dremel 8050-N/18
Guide Cannula Plastics one 326OPG/spc
Injection Cannula Plastics one C315I/spc
Dummy Cannula Plastics one C315DC/spc
Suture thread coated vicryl rapide 4-0 Ethicon VR2297
Dental Acrylic Cement Harvard Apparatus 72-6906
Screws JI Morris Company P0090CE125
6E10 antibody (mouse IgG1 isotype) BioLegend 803003
Mouse IgG1 isotype control antibody Abcam AB18447
Alzet osmotic pumps model 1007D Durect corporation 290
Isoflurane Baxter CA2L9100
Amyloid-beta 1-42 rPeptide A-1163-1
Hamilton syringe (10 µL) Fisher Scientique 14815279
Infusion Syringe Pump CMA 402 Harvard Apparatus CMA8003110
Syringe 1 mL BD 309659
Needle 21G Terumo NN-2125R
Snuggle Lomir Biomedical RTS04

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Citer Cet Article
Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus. J. Vis. Exp. (Pending Publication), e22127, doi: (2024).

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