Source: Zychlinsky Scharff, A. et al., Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice. J. Vis. Exp. (2017)
This video demonstrates the establishment of uropathogenic Escherichia coli infection in a female mouse bladder through transurethral catheter instillation. The injected bacteria successfully establish a urinary tract infection within the bladder, providing a valuable platform for studying healthcare-associated diseases related to the urinary tract.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Catheters
2. Preparation of Uropathogenic Escherichia coli for Infection
3. Preparation of Mice
4. Transurethral Instillation of Female Mice
The authors have nothing to disclose.
BD Insyte Autoguard Shielded IV Catheters 24G, 0.7 mm external diameter, 14 mM long | BD Medical | 381811 or 381411* | * catalog number is country-dependent |
Inoculating loop | Greiner Bio-One | 731171 | |
LB Miller broth | Difco | 244620 | |
LB Miller agar | Difco | 244520 | |
Cuvettes | Bio-rad | 223-9955 | |
Syringes | B Braun | 9166017V | |
Insulin syringe | Terumo | BS05M2913 |