Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy

Published: October 31, 2023

Abstract

Source: Anderson, L. S. et al., A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection. J. Vis. Exp. (2019)

This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Mouse Source and Housing Derive Lysozyme M- enhanced green fluorescent protein (LysM-eGFP) mice on a C57BL/6J genetic background as described previously. Derive LysM-EGFP× myeloid differentiation primary response 88 (MyD88)-/- mice by crossing LysM-EGFP mice with MyD88-/- mice on a C57BL/6J background. Ho…

Divulgations

The authors have nothing to disclose.

Materials

6mm Disposable Biopsy Punch Integra Miltex 33-36
Buprenoprhine hydrochloride injectable Western Medical Supply 7292 0.3 mg/mL
C57BL/6J Mice Jackson Labratory 664
LysM-eGFP Mice Thomas Graff Albert Einstein College of New York NA
MyD88 KO Mice Jackson Labratory 9088
Non-woven sponges AMD- Ritmed Inc A2101-CH 5 cm x 5 cm
Povidone Iodine 10% Solution Aplicare 697731

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Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy. J. Vis. Exp. (Pending Publication), e21659, doi: (2023).

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