Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy
Published: October 31, 2023
Abstract
Source: Anderson, L. S. et al., A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection. J. Vis. Exp. (2019)
This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Mouse Source and Housing Derive Lysozyme M- enhanced green fluorescent protein (LysM-eGFP) mice on a C57BL/6J genetic background as described previously. Derive LysM-EGFP× myeloid differentiation primary response 88 (MyD88)-/- mice by crossing LysM-EGFP mice with MyD88-/- mice on a C57BL/6J background. Ho…
Divulgations
The authors have nothing to disclose.
Materials
| 6mm Disposable Biopsy Punch | Integra Miltex | 33-36 | |
| Buprenoprhine hydrochloride injectable | Western Medical Supply | 7292 | 0.3 mg/mL |
| C57BL/6J Mice | Jackson Labratory | 664 | |
| LysM-eGFP Mice | Thomas Graff Albert Einstein College of New York | NA | |
| MyD88 KO Mice | Jackson Labratory | 9088 | |
| Non-woven sponges | AMD- Ritmed Inc | A2101-CH | 5 cm x 5 cm |
| Povidone Iodine 10% Solution | Aplicare | 697731 |
Tags
Citer Cet Article
Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy. J. Vis. Exp. (Pending Publication), e21659, doi: (2023).