Source: Gayle, P. et al., Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses. J. Vis. Exp. (2019).
This video demonstrates an in vitro quantification technique to determine Listeria monocytogenes infection of macrophages by the colony-forming unit assay. The bacterial colonies on the agar plate represent the intracellular bacterial population that escapes the host-defense mechanism and survives within the macrophages.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparing Cells for Infection
2. Preparing Listeria monocytogenes (Lm) for infection
3. Infection
4. Sampling Processing and Analysis of Intracellular and Emergent Lm by CFU Assay
The authors have nothing to disclose.
BHI (Brain Heart Infusion) broth | EMD Milipore | 110493 | |
DMEM media | Gibco | 11965-092 | |
FBS – Heat-Inactivated | Sigma-Aldrich | F4135-500ML | |
LB agar | Grow Cells MS | MBPE-4040 | |
TPP Tissue Culture 48-Well Plates | MIDSCI | TP92048 |