Source: Maier, I. Engineering Antiviral Agents via Surface Plasmon Resonance. J. Vis. Exp. (2022)
This video describes the surface plasmon resonance, or SPR, technique for studying the interaction between two molecules using a sensor chip. The change in resonance angle and the intensity of the reflected light are detected as a function of the association and dissociation of two molecules, and this provides information about the interactions between the two molecules.
The authors have nothing to disclose.
Äkta primeplus | Cytiva | ||
Amicon tubes | Merck | C7715 | |
Ampillicin | Sigma-Aldrich | A5354 | |
Beckmann Coulter Cooler Allegra X-30R centrifuge | Beckman Coulter | B06320 | |
Cell spreader | Sigma-Aldrich | HS86655 | Silver stainless steel, bar L 33 mm |
Custom DNA Oligos | Sigma-Aldrich | OLIGO | |
Custom Gensynthesis | GenScript | #1390661 | Cloning vector: pET27b(+) |
Cytiva HBS-EP+ Buffer 10, 4x50mL | Thermo Scientific | 50-105-5354 | |
Dionex UlitMate 3000 | Thermo Scientific | IQLAAAGABHFAPBMBFB | |
Dpn I restriction enzyme (10 U/μL) | Fisher Scientific | ER1701 | |
DTT | Merck | DTT-RO | |
EDC | Merck | 39391 | |
EDTA | Merck | E9884 | |
Eppendorf Safe-Lock Tubes | Eppendorf | 30120086 | |
Eppendorf Safe-Lock Tubes | Eppendorf | 30120094 | |
Eppendorf Minispin and MiniSpin Plus personal microcentrifuge | Sigma-Aldrich | Z606235 | |
Ethanol | Merck | 51976 | |
Ethanolamine HCl | Merck | E6133 | |
Falcon 50mL Conical Centrifuge Tubes | Fisher Scientific | 14-432-22 | |
Falcon 14 mL Round Bottom Polystyrene Test Tube, with Snap Cap, Sterile, 25/Pack | Corning | 352057 | |
Glucose | Merck | G8270 | |
Glycine HCl | Merck | 55097 | |
HA H3 protein | Abcam | ab69751 | |
HEPES | Merck | H3375 | |
His-select Ni2+ | Merck | H0537 | |
Imidazole | Merck | I2399 | |
IPTG | Merck | I6758 | |
Kanamycin A | Sigma-Aldrich | K1377 | |
Kromasil 300-5-C4 | Nouryon | ||
LB agar | Merck | 52062 | |
LB agar | Merck | 19344 | |
LB Lennox | Merck | L3022 | |
Lysozyme | Merck | 10837059001 | |
Magnesium chloride | Merck | M8266 | |
Magnesium sulfate | Merck | M7506 | |
NaH2P04 | Merck | S0751 | |
NanoDrop UV-Vis2000c spectrophotometer | Thermo Scientific | ND2000CLAPTOP | |
NaOH | Merck | S5881 | |
NHS | Merck | 130672 | |
NZ amine (casein hydrolysate) | Merck | C0626 | |
PBS | Merck | 806552 | |
PD MidiTrap G-10 | Sigma-Aldrich | GE28-9180-11 | |
Peptone | Merck | 70171 | |
pET11a | Merck Millipore (Novagen) | 69436 | |
PMSF | Merck | PMSF-RO | |
QIAprep Spin Miniprep Kit (1000) | Qiagen | 27106X4 | |
Reichert Software Package Autolink1-1-9 | Reichert | ||
Reichert SPR SR7500DC Dual Channel System | Reichert | ||
Scrubber2-2012-09-04 for data analysis | Reichert | ||
SDS | Merck | 11667289001 | |
Site-directed mutagenesis kit incl pUC18 control plasmid | Stratagene | #200518 | |
Sodim chloride | Merck | S9888 | |
Sodium acetate Trihydrate | Merck | 236500 | |
SPR sensor chip C19RBDHC30M | XanTec bioanalytics | SCR C19RBDHC30M | |
SPR sensor chip CMD500D | XanTec bioanalytics | SCR CMD500D | |
Sterilin Standard 90mm Petri Dishes | Thermo Scientific | 101R20 | |
TBS | Merck | T5912 | 10x, solution |
Triton-X100 | Merck | T8787 | |
Tryptone | Merck | 93657 | |
Tween20 | Merck | P1379 | |
Vortex-Genie 2 Mixer | Merck | Z258423 | |
X-gal | Merck | XGAL-RO | |
XL1-Blue Supercompetent Cells | Stratagene | #200236 | |
Yeast extract | Merck | Y1625 |