Source: Wong, A. Y. H. et al. Fluorescent Silver Staining of Proteins in Polyacrylamide Gels. J. Vis. Exp. (2019)
In this video, we demonstrate a silver staining method of polyacrylamide gels for the visualization of total protein by using fluorogenic probes.
1. Preparation of the Gel
2. Fixation of the Gel
3. Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
4. Fluorogenic Development of the Gel
5. Destaining and Imaging
The authors have nothing to disclose.
LDS Sample Buffer (4X) | Thermo Fisher Scientific | NP0007 | Reagent |
4-12% Bis-Tris Protein Gels, 1.0 mm, 15-well | Thermo Fisher Scientific | NP0323BOX | Precast gel |
Sample Reducing Agent (10X) | Thermo Fisher Scientific | NP0004 | Reagent |
MES SDS Running Buffer (20X) | Thermo Fisher Scientific | NP0002 | Reagent |
Mini Gel Tank | Thermo Fisher Scientific | A25977 | Equipment |
300W Power Supply (230 VAC) | Thermo Fisher Scientific | PS0301 | Equipment |
Unstained Protein Ladder | Thermo Fisher Scientific | 26614 | Sample |
Silver nitrate | Sigma-Aldrich | 31630-25G-R | Reagent |
Ethanol | Bragg and co. | 42520J | Reagent |
Acetic acid | J.T. Baker | 103201A | Reagent |
Milli-Q Synthesis A10 | Merk | – | Provides 18.2 MΩ.cm water |
Gel documentation system (c600 model) | Azure biosystems | – | Equipment |