CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
Published: April 30, 2023
Abstract
Source: Hubner, E. K., et al. Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection. J. Vis. Exp. (2018)
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Induction of Transfected CreER with Tamoxifen CAUTION: Tamoxifen is harmful, may be cancerous or damage fertility. Please refer to the safety data sheet. Plan intraperitoneal tamoxifen injections on three consecutive days. On day 1, dissolve 10 mg of tamoxifen in 40…
Divulgations
The authors have nothing to disclose.
Materials
| Injekt Syringe F 1 ml | Braun | 9166017V | For intraperitoneal injection |
| Tamoxifen | Sigma-Aldrich | T5648-1G | For CreER activation |
| Corn oil | Sigma-Aldrich | C8267-500ML | Carrier for tamoxifen injections |
Tags
Citer Cet Article
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model. J. Vis. Exp. (Pending Publication), e20976, doi: (2023).