Orthotopic Porcine Kidney Transplantation: A Technique to Transplant Graft Kidney in Porcine Model

Published: April 30, 2023

Abstract

Source: Liu, W. J. et al., Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation and Continuous Telemetry. J. Vis. Exp. (2020).

This video describes orthotopic kidney auto-transplantation in a porcine model. This surgical procedure involves the transplantation of an animal's kidney graft at its original anatomical location. The model is used to investigate new treatments for end-stage kidney diseases in humans.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Basic techniques and common procedures Use female German landrace pigs (or comparable) for this protocol. Figure 1 depicts the summary of the described experimental protocol. Deliver the animals to the research facility 14 days before the surgery for acclimatization and house them in a temperature- and humidity-controll…

Representative Results

Figure 1: Study flowchart and protocol. Abbreviations used: POD-postoperative day; ECG-electrocardiography. Figure 2: Animal housing facility with real-time and continuous telemetry monitoring of up to 6 animals. </…

Divulgations

The authors have nothing to disclose.

Materials

Anesthesia materials, drugs and medications
Atropine sulfate solution for injection, 100mg Dr. Franz Köhler Chemie GmbH, Bensheim, Germany 1821288 parasympatholytic agent, premedication
Bepanthen ointment for eyes and nose Bayer Vital AG, Leverkusen, Germany 1578675 eye ointment
BD Discardit II syringes, 2ml, 5ml, 10ml,20ml Becton Dickinson GmbH, Heidelberg, Germany 300928, 309050,309110, 300296  syringes
BD Micolance 3 (20G yellow) Cannula Becton Dickinson GmbH, Heidelberg, Germany 305888  venous catheter
BD Venflon Pro Safety (20G pink)  Becton Dickinson GmbH, Heidelberg, Germany 4491101 venous catheter
Buprenorphine (Buprenovet)  Bayer Vital AG, Leverkusen, Germany 794-996  analgesia
Cefuroxime 750mg, powder for preparing injection solution FRESENIUS KABI Deutschland GmbH, Bad Homburg, Germany J01DC02  antibiotics
Covidien Hi-Contour, Endotracheal Tube 7,5 with Cuffed Murphy Eye Covidien Deutschland GmbH,Neustadt/Donau, Germany COV-107-75E  endotracheal Tube
FENTANYL 0,5 mg Rotexmedica solution for injection Rotexmedica GmbH Arzneimittelwerk, Trittau, Germany 4993593 opioide analgetic agent
Furosemide-ratiopharm 250 mg/25 ml solution for injection Ratiopharm GmbH, Ulm, Germany  1479542 loop diuretics
Glucose 5% solution for infusion (500ml, 250ml) B. Braun Deutschland GmbH & Co. KG, Melsungen, Germany 3705273, 03705422 infusion fluid
Glucose 20% solution for infusion  B. Braun Deutschland GmbH & Co. KG, Melsungen, Germany 4164483 osmotic diuresis
Heparin-Sodium 5000 I.E./ml  B. Braun Deutschland GmbH & Co. KG, Melsungen, Germany 15782698 anticoagulant
Isoflurane-Piramal (Isoflurane)  Piramal Critical Care Deutschland GmbH, Hallbergmoos, Germany 9714675 volatile anaesthetic agent
Ketamine (Ketamine hydrochloride) 10% Medistar Arzneimittelvertrieb GmbH, Ascheberg, Germany 4230 general anaestetic agent
MIDAZOLAM 15mg/3ml  Rotexmedica GmbH, Arzneimittelwerk, Trittau, Germany 828093 hypnotica, sedative agent
NaCl 0,9% solution for infusion (500ml,1000ml) B. Braun Deutschland GmbH & Co. KG, Melsungen, Germany 864671.8779 infusion fluid
Norepinephrine (Arterenol)  Sanofi-Aventis Deutschland GmbH, Frankfurt, Germany 16180  increase in blood pressure
Pantoprazole 40mg/solution for injection Laboratorios Normon,Madrid, Spain  11068  proton pump inhibitor
Paveron N 25mg/ml solution for injection (Papaverine Hydrochloride) LINDEN Arzneimittel-Vertrieb-GmbH, Heuchelheim, Germany 2748990 spasmolytic agent for vasodilatation
Propofol 1% (10mg/ml) MCT Fresenius FRESENIUS KABI Deutschland, GmbH, Bad Homburg, Germany 654210 general anaesthetic agent
Ringer solution  B. Braun Deutschland GmbH & Co. KG, Melsungen, Germany 1471411  infusion fluid
Urine catheter ruffle 12CH  Wirutec Rüsch Medical Vertriebs GmbH, Sulzbach, Germany RÜSCH-180605-12  transurethral urine catheter
Surgical materials
Appose ULC Skin Stapler  Covidien Deutschland GmbH,Neustadt/Donau, Germany 8886803712 skin stapler
Feather Disposable Scapel (11)(21)  Feather, Japan  8902305.395 scapel
Prolene 2-0, blue monofil VISIBLACK, FS needle Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany EH7038H  skin
Prolene 5-0 (simply angulated, C1 needle) blue monofil VISI-BLACK Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany EH7227H  vascular
Prolene 5-0 (double armed, C1 needle) 60cm Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany KBB5661H  vascular
Prolene 6-0 (double armed, C1 needle) 60cm Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany EH7228H vascular
Sempermed derma PF Surgical Gloves Seril Gr. 7, 7.5, 8 Semperit investment Asia Pte Ltd, Singapore 4200782, 4200871, 4200894  surgical gloves
Sentinex® PRO Surgical Gowns Spunlace XL 150cm Lohmann & Rauscher GmbH & Co. KG, Neuwied, Germany 19302 surgical gown
Telasorp Belly wipes (green 45x45cm) PAUL HARTMANN AG, Heidenheim, Germany 4542437  abdominal towel
Pediatric urine catheter  Uromed Kurt Drews KG, Oststeinbeck, Germany PZN 03280856  used for the uretero-cutaneus stoma
VICRYL- 0 MH Plus  Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany V324  fascial closure
VICRYL – 3-0, SH1 Plus needle, 75cm Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany W9114  subcutaneous suture, peritoneal suture
VICRYL – 3-0, SH1 Plus needle, 75cm Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany V780  subcutaneous suture, peritoneal suture
VICRYL – ligatures Sutupak purple braided, 3-0 Johnson & Johnson Medical GmbH- Ethicon Deutschland, Norderstedt, Germany V1215E  threads for ligature
3M™ Standard Surgical Mask 1810F  3M Deutschland GmbH, Neuss, Germany 3M-ID 7000039767  surgical mask
Surgical instruments
Anatomical forceps Standard  ASANUS Medizintechnik GmbH, Tuttlingen, Germany PZ0260  anatomical forceps
Atraumatic tweezers steel, De Bakey Tip 1,5mm 8" ASANUS Medizintechnik GmbH, Tuttlingen, Germany GF0840  anatomical atraumatic forceps
Bipolar forceps 16 cm straight, Branch 0,30 mm pointed, universal fit Bühler Instrumente Medizintechnik GmbH,Tuttlingen, Germany 08/0016-A  biopolar forceps
Bulldog clamp atraumatic, curved, De bakey 78 mm, 3" ASANUS Medizintechnik GmbH, Tuttlingen, Germany GF0900  bulldog clamps
DE BAKEY-SATINSKY vascular clamp 215mm ASANUS Medizintechnik GmbH, Tuttlingen, Germany GF1661  vascular clamp
Dissecting scissors Mayo, 250 mm, 10" ASANUS Medizintechnik GmbH, Tuttlingen, Germany SC2232  Scissors for dissection
Dissecting scissors Metzenbaum-Fino, 260 mm, 101/4" ASANUS Medizintechnik GmbH, Tuttlingen, Germany SC2290  Scissors for dissection
Draeger CATO Anesthetic machine with PM8050 Monitor Dräger, Drägerwerk AG & Co. KGaA, Lübeck, Germany 106782  Ventilation System
Fine Tweezers, ADSON 180 mm  ASANUS Medizintechnik GmbH, Tuttlingen, Germany ADSONPZ0571  fine forceps
Gosset abdomenal wall spreader  CHIRU-INSTRUMENTE, Kaierstuhl, Germany 09-621512  abdominal retractor
Microsurgical/watermaker tweezers LINZ 150mm 6" Ergo round handle ASANUS Medizintechnik GmbH, Tuttlingen, Germany MN0087  fine microsurgical forceps
Surgical forceps Standard 5 3/4"  ASANUS Medizintechnik GmbH, Tuttlingen, Germany PZ1260  surgical forceps
Surgical scissors standard pointed-blunt (thread/cloth scissors)175 mm, 7" ASANUS Medizintechnik GmbH, Tuttlingen, Germany SC1522  surgical scissors
Further material
Heating pad  Eickemeyer – Medizintechnik für Tierärzte KG, Tuttlingen, Germany 648050 MHP-E1220  maintain body temperature during surgery
Laryngoscope, customized  Wittex GmbH, Simbach, Germany  333222230 expose the vocal cord
Rectal temperature probe  Asmuth Medizintechnik, Minden, Germany ASD-RA4  measure body temperature
Ultrasound device, Sonosite Edge-II  FUJIFILM SonoSite GmbH, Frankfurt, Germany V21822  ultrasound and color Doppler
Urine bag 2000ml Volume  ASID BONZ GmbH, Herrenberg, Germany 2062578 disposable urine bag connected to the uretero-cutaneous fistula catheter

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Citer Cet Article
Orthotopic Porcine Kidney Transplantation: A Technique to Transplant Graft Kidney in Porcine Model. J. Vis. Exp. (Pending Publication), e20914, doi: (2023).

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