Source: Pacheco-Baltazar, A., et al., Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).
This video shows an ex vivo technique for isolated lung perfusion. It is a tool for pulmonary research that will aid studies of various physiological and pathological mechanisms involved in lung transplantation procedures.
The authors have nothing to disclose.
2-Stop Tygon E-Lab Tubing, 3.17 mm ID, 12/pack, Black/White | Hugo Sachs Elektronik (HSE) | 73-1864 | |
Adapter for Positive Pressure Ventilation on IPL-4 | Hugo Sachs Elektronik (HSE) | 73-4312 | |
Adapter for Positive Pressure Ventilation on IPL-4 | Hugo Sachs Elektronik (HSE) | 73-4312 | |
Alternative Pressure-Free Gas Supply for IPL-4: To supply the trachea with gas mixture different from room air during negative ventilation | Hugo Sachs Elektronik (HSE) | 73-4309 | |
Base Unit for the Rabbit to Fetal Pig Isolated Perfused Lung | Hugo Sachs Elektronik (HSE) | 73-4138 | |
Bovine serum A2:D41albumin lyophilized powder | sigma | 3912 | 500 g |
Calcium chloride, CaCl2·2H2O. | JT Baker | 10035-04-8 | |
Cryogenic vials | Corning | 430659 | 2 mL |
D-glucose, C6H12O6. | sigma | G5767 | |
Differential Low Pressure Transducer DLP2.5, Range +- 2.5 cmH2O, HSE Connector | Hugo Sachs Elektronik (HSE) | 73-3882 | |
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector | Hugo Sachs Elektronik (HSE) | 73-0064 | |
Eppendorf tubes | |||
Ethanol absolute HPLC grade | Caledon | ||
Falcon tubes | 14 mL | ||
Harvard Peristaltic Pump P-230 (Complete with Control Box and P-230 Motor Drive) | Hugo Sachs Elektronik (HSE) | 70-7001 | |
Heated Linear Pneumotachometer 0 to 10 L/min flow range | Hugo Sachs Elektronik (HSE) | 59-9349 | |
Heater Controller for Single Pneumotachometer 230 VAC, 50 Hz | Hugo Sachs Elektronik (HSE) | 59-9703 | |
Heparin | PISA | 5000 UI | |
HPLC Column (C18 100A 5U) | Alltech | 98121213 | 150 mm x 4.6 mm |
Hydrophilic Syringe Filter | Millex | SLLGR04NL | 4 mm |
IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 | Hugo Sachs Elektronik (HSE) | 73-4296 | |
IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 V | Hugo Sachs Elektronik (HSE) | 73-4296 | |
Jacketed Glass Reservoir for Buffer Solution, with Frit and Tubing, 6.0 L | Hugo Sachs Elektronik (HSE) | 73-0322 | |
Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C | Hugo Sachs Elektronik (HSE) | 73-0125 | |
Left Atrium Cannula for Rabbit with Basket, OD 5.9 mm | Hugo Sachs Elektronik (HSE) | 73-4162 | |
Low Range Blood Pressure Transducer P75 for PLUGSYS Module | Hugo Sachs Elektronik (HSE) | 73-0020 | |
Magnesium sulfate heptahydrate, MgSO4·7H2O | JT Baker | 10034-99-8 | |
Microcentrifuge Tube | Corning | 430909 | |
Negative Pressure Ventilation Control Option with Pressure Regulator for IPL-4 | Hugo Sachs Elektronik (HSE) | 73-4298 | |
New Zeland rabbits | |||
PISABENTAL (Pentobarbital sodium) | PISA | Q-7833-215 | |
PLUGSYS Case, Type 603* 7 | Hugo Sachs Elektronik (HSE) | 73-0045 | |
PLUGSYS TCM Time Counter Module | Hugo Sachs Elektronik (HSE) | 73-1750 | |
PLUGSYS Transducer Amplifier Module (TAM-A) | Hugo Sachs Elektronik (HSE) | 73-0065 | |
PLUGSYS Transducer Amplifier Module (TAM-D) | Hugo Sachs Elektronik (HSE) | 73-1793 | |
PLUGSYS VCM-4R Ventilation Control Module with Pressure Regulator | Hugo Sachs Elektronik (HSE) | 73-1755 | |
Potassium chloride, KCl. | JT Baker | 3040-01 | |
Potassium dihydrogen phosphate, KH2PO4 | JT Baker | 7778-77-0 | |
Pulmonary Artery Cannula for Rabbit with Basket, OD 4.6 mm | Hugo Sachs Elektronik (HSE) | 73-4161 | |
Servo Controller for Perfusion (SCP | Hugo Sachs Elektronik (HSE) | 73-2806 | |
Snap Cap Microcentrifuge Tube | Costar | 3620 | 1.7 mL |
Sodium bicarbonate, NaHCO3 | sigma | S6014 | |
Sodium chloride, NaCl. | sigma | S9888 | |
Surgical gloves No. 7 1/2 | |||
Surgical gloves No. 8 | |||
Taygon tubes | Masterflex | ||
Tracheal Cannula for Rabbit, OD 5.0 mm | Hugo Sachs Elektronik (HSE) | 73-4163 |